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TOB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TOB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101414-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TOB2 (NM_016272), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BK223H9 | KIAA1663 | Protein Tob4 | TROB2 | TOB2 | TOB4 | TOBL | Transducer of erbB-2 2 | Protein Tob2 | Transducer of ERBB2 | 2
Origin Species
Human
NCBI GenBank
NM_016272
SwissProt
Q14106

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
36.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQLEIKVALN FIISYLYNKL PRRRADLFGE ELERLLKKKY EGHWYPEKPL KGSGFRCVHI
61GEMVDPVVEL AAKRSGLAVE DVRANVPEEL SVWIDPFEVS YQIGEKGAVK VLYLDDSEGC
121GAPELDKEIK SSFNPDAQVF VPIGSQDSSL SNSPSPSFGQ SPSPTFIPRS AQPITFTTAS
181FAATKFGSTK MKKGGGAASG GGVASSGAGG QQPPQQPRMA RSPTNSLLKH KSLSLSMHSL
241NFITANPAPQ SQLSPNAKEF VYNGGGSPSL FFDAADGQGS GTPGPFGGSG AGTCNSSSFD
301MAQVFGGGAN SLFLEKTPFV EGLSYNLNTM QYPSQQFQPV VLAN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

344 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.