Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TOB1 / TOB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100637-100
Catalog Number: LS-G100637
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TOB(TOB1) (NM_005749), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APRO6 | Protein Tob1 | Transducer of ERBB2 | 1 | TROB | TOB | PIG49 | Transducer of erbB-2 1 | Proliferation-inducing gene 49 | TOB1 | TROB1
- Origin Species
- Human
- NCBI GenBank
- NM_005749
- SwissProt
- P50616
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQLEIQVALN FIISYLYNKL PRRRVNIFGE ELERLLKKKY EGHWYPEKPY KGSGFRCIHI61GEKVDPVIEQ ASKESGLDID DVRGNLPQDL SVWIDPFEVS YQIGEKGPVK VLYVDDNNEN121GCELDKEIKN SFNPEAQVFM PISDPASSVS SSPSPPFGHS AAVSPTFMPR STQPLTFTTA181TFAATKFGST KMKNSGRSNK VARTSPINLG LNVNDLLKQK AISSSMHSLY GLGLGSQQQP241QQQQQPAQPP PPPPPPQQQQ QQKTSALSPN AKEFIFPNMQ GQGSSTNGMF PGDSPLNLSP301LQYSNAFDVF AAYGGLNEKS FVDGLNFSLN NMQYSNQQFQ PVMANTRTRPLEQKLISEEDLAANDILDYKDDDDKV
345 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.