Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TNXB / Tenascin XB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108797-100
Catalog Number: LS-G108797
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TNXB (NM_032470), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EDS3 | Hexabrachion-like protein | Growth-inhibiting protein 45 | Tenascin-XB | TENX | Tenascin XB | TN-X | TNXB1 | TNXBS | XB | TNX | Tenascin XB1 | Tenascin XB2 | Tenascin-X | TNXB | XBS | HXBL | TNXB2
- Origin Species
- Human
- SwissProt
- P22105
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRLSWSVAQG PFDSFVVQYE DTNGQPQALL VDGDQSKILI SGLEPSTPYR FLLYGLHEGK61RLGPLSAEGT TGLAPAGQTS EESRPRLSQL SVTDVTTSSL RLNWEAPPGA FDSFLLRFGV121PSPSTLEPHP RPLLQRELMV PGTRHSAVLR DLRSGTLYSL TLYGLRGPHK ADSIQGTART181LSPVLESPRD LQFSEIRETS AKVNWMPPPS RADSFKVSYQ LADGGEPQSV QVDGQARTQK241LQGLIPGARY EVTVVSVRGF EESEPLTGFL TTVPDGPTQL RALNLTEGFA VLHWKPPQNP301VDTYDIQVTA PGAPPLQAET PGSAVDYPLH DLVLHTNYTA TVRGLRGPNL TSPASITFTT361GLEAPRDLEA KEVTPRTALL TWTEPPVRPA GYLLSFHTPG GQTQEILLPG GITSHQLLGL421FPSTSYNARL QAMWGQSLLP PVSTSFTTGG LRIPFPRDCG EEMQNGAGAS RTSTIFLNGN481RERPLIVFCD METDGGGWLV FQRRMDGQTD FWRDWEDYAH GFGNISGEFW LGNEALHSLT541QAGDYSMRVD LRAGDEAVFA QYDSFHVDSA AEYYRLHLEG YHGTAGDSMS YHSGSVFSAR601DRDPNSLLIS CAVSYRGAWW YRNCHYANLN GLYGSTVDHQ GVSWYHWKGF EFSVPFTEMK661LRPRNFRSPA GGGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
673 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.