LSBio
TNS4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TNS4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108593-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TNS4 (NM_032865), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C terminal tensin like | C-terminal tensin-like protein | CTEN | PP14434 | Tensin 4 | Tensin-4 | TNS4
Origin Species
Human
NCBI GenBank
NM_032865
SwissProt
Q8IZW8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQVMSSPLL AGGHAVSLAP CDEPRRTLHP APSPSLPPQC SYYTTEGWGA QALMAPVPCM
61GPPGRLQQAP QVEAKATCFL PSPGEKALGT PEDLDSYIDF SLESLNQMIL ELDPTFQLLP
121PGTGGSQAEL AQSTMSMRKK EESEALDIKY IEVTSARSRC HDGPQHCSSP SVTPPFGSLR
181SGGLLLSRDV PRETRSSSES LIFSGNQGRG HQRPLPPSEG LSPRPPNSPS ISIPCMGSKA
241SSPHGLGSPL VASPRLEKRL GGLAPQRGSR ISVLSASPVS DVSYMFGSSQ SLLHSSNSSH
301QSSSRSLESP ANSSSSLHSL GSVSLCTRPS DFQAPRNPTL TMGQPRTPHS PPLAKEHASS
361CPPSITNSMV DIPIVLINGC PEPGSSPPQR TPGHQNSVQP GAASPSNPCP ATRSNSQTLS
421DAPFTTCPEG PARDMQPTMK FVMDTSKYWF KPNITREQAI ELLRKEEPGA FVIRDSSSYR
481GSFGLALKVQ EVPASAQSRP GEDSNDLIRH FLIESSAKGV HLKGADEEPY FGSLSAFVCQ
541HSIMALALPC KLTIPQRELG GADGASDSTD SPASCQKKSA GCHTLYLSSV SVETLTGALA
601VQKAISTTFE RDILPTPTVV HFKVTEQGIT LTDVQRKVFF RRHYPLTTLR FCGMDPEQRK
661WQKYCKPSWI FGFVAKSQTE PQENVCHLFA EYDMVQPASQ VIGLVTALLQ DAERM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

715 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.