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TNRC6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TNRC6B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TNRC6B antibodies →
$504.00each
Catalog Number: LS-G121086-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TNRC6B (NM_001024843), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1093 | TNRC6B
Origin Species
Human
NCBI GenBank
N64803
SwissProt
Q9UPQ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQTNEGEVSE ESSSKVEQED FVMEGHGKTP PPGEESKQEK EQEREEQLME DKKRKKEDKK
61KKEATQKVTE QKTKVPEVTK PSLSQPTAAS PIGSSPSPPV NGGNNAKRVA VPNGQPPSAA
121RYMPREVPPR FRCQQDHKVL LKRGQPPPPS CMLLGGGAGP PPCTAPGANP NNAQVTGALL
181QSESGTAPVW SKSTPPAPDN GTSAWGEPNE SSPGWGEMDD TGASTTGWGN TPANAPNAMK
241PNSKSMQDGW GESDGPVTGA RHPSWEEEED GGVWNTTGSQ GSASSHNSAS WGQGGKKQMK
301CSLKGGNNDS WMNPLAKQFS NMGLLSQTED NPSSKMDLSV GSLSDKKFDV DKRAMNLGDF
361NDIMRKDRSG FRPPNSKDMG TTDSGPYFEK GGSHGLFGNS TAQSRGLHTP VQPLNSSPSL
421RAQVPPQFIS PQVSASMLKQ FPNSGLSPGL FNVGPQLSPQ QIAMLSQLPQ IPQFQLACQL
481LLQQQQQQQL LQNQRKISQA VRQQQEQQLA RMVSALQQQQ QQQQRQPGMK HSPSHPVGPK
541PHLDNMVPNA LNVGLPDLQT KGPIPGYGSG FSSGGMDYGM VGGKEAGTES RFKQWTSMME
601GLPSVATQEA NMHKNGAIVA PGKTRGGSPY NQFDIIPGDT LGGHTGPAGD SWLPAKSPPT
661NKIGSKSSNA SWPPEFQPGV PWKGIQNIDP ESDPYVTPGS VLGGTATSPI VDTDHQLLRD
721NTTGSNSSLN TSLPSPGAWP YSASDNSFTN VHSTSAKFPD YKSTWSPDPI GHNPTHLSNK
781MWKNHISSRN TTPLPRPPPG LTNPKPSSPW SSTAPRSVRG WGTQDSRLAS ASTWSDGGSV
841RPSYWLVLHN LTPQIDGSTL RTICMQHGPL LTFHLNLTQG TALIRYSTKQ EAAKAQTALH
901MCVLGNTTIL AEFATDDEVS RFLAQAQPPT PAATPSAPAA GWQSLETGQN QSDPVGPALN
961LFGGSTGLGQ WSSSAGGSSG ADLAGASLWG PPNYSSSLWG VPTVEDPHRM GSPAPLLPGD
1021LLGGGSDSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1029 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.