Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TNR / Tenascin R Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117445-100
Catalog Number: LS-G117445
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TNR (NM_003285), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Janusin | Tenascin-R | TN-R | TNR | Restrictin | Tenascin R
- Origin Species
- Human
- NCBI GenBank
- NM_003285
- SwissProt
- Q92752
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 149.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGADGETVVL KNMLIGVNLI LLGSMIKPSE CQLEVTTERV QRQSVEEEGG IANYNTSSKE61QPVVFNHVYN INVPLDNLCS SGLEASAEQE VSAEDETLAE YMGQTSDHES QVTFTHRINF121PKKACPCASS AQVLQELLSR IEMLEREVSV LRDQCNANCC QESAATGQLD YIPHCSGHGN181FSFESCGCIC NEGWFGKNCS EPYCPLGCSS RGVCVDGQCI CDSEYSGDDC SELRCPTDCS241SRGLCVDGEC VCEEPYTGED CRELRCPGDC SGKGRCANGT CLCEEGYVGE DCGQRQCLNA301CSGRGQCEEG LCVCEEGYQG PDCSAVAPPE DLRVAGISDR SIELEWDGPM AVTEYVISYQ361PTALGGLQLQ QRVPGDWSGV TITELEPGLT YNISVYAVIS NILSLPITAK VATHLSTPQG421LQFKTITETT VEVQWEPFSF SFDGWEISFI PKNNEGGVIA QVPSDVTSFN QTGLKPGEEY481IVNVVALKEQ ARSPPTSASV STVIDGPTQI LVRDVSDTVA FVEWIPPRAK VDFILLKYGL541VGGEGGRTTF RLQPPLSQYS VQALRPGSRY EVSVSAVRGT NESDSATTQF TTEIDAPKNL601RVGSRTATSL DLEWDNSEAE VQEYKVVYST LAGEQYHEVL VPRGIGPTTR ATLTDLVPGT661EYGVGISAVM NSQQSVPATM NARTELDSPR DLMVTASSET SISLIWTKAS GPIDHYRITF721TPSSGIASEV TVPKDRTSYT LTDLEPGAEY IISVTAERGR QQSLESTVDA FTGFRPISHL781HFSHVTSSSV NITWSDPSPP ADRLILNYSP RDEEEEMMEV SLDATKRHAV LMGLQPATEY841IVNLVAVHGT VTSEPIVGSI TTGIDPPKDI TISNVTKDSV MVSWSPPVAS FDYYRVSYRP901TQVGRLDSSV VPNTVTEFTI TRLNPATEYE ISLNSVRGRE ESERICTLVH TAMDNPVDLI961ATNITPTEAL LQWKAPVGEV ENYVIVLTHF AVAGETILVD GVSEEFRLVD LLPSTHYTAT1021MYATNGPLTS GTISTNFSTL LDPPANLTAS EVTRQSALIS WQPPRAEIEN YVLTYKSTDG1081SRKELIVDAE DTWIRLEGLL ENTDYTVLLQ AAQDTTWSSI TSTAFTTGGR VFPHPQDCAQ1141HLMNGDTLSG VYPIFLNGEL SQKLQVYCDM TTDGGGWIVF QRRQNGQTDF FRKWADYRVG1201FGNVEDEFWL GLDNIHRITS QGRYELRVDM RDGQEAAFAS YDRFSVEDSR NLYKLRIGSY1261NGTAGDSLSY HQGRPFSTED RDNDVAVTNC AMSYKGAWWY KNCHRTNLNG KYGESRHSQG1321INWYHWKGHE FSIPFVEMKM RPYNHRLMAG RKRQSLQFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1358 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.