Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TNPO2 / Importin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G125685-100
Catalog Number: LS-G125685
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TNPO2 (NM_001136196), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Transportin 2 | TRN2 | TNPO2 | Transportin-2 | Importin 3 | IPO3 | Karyopherin beta-2b | KPNB2B
- Origin Species
- Human
- NCBI GenBank
- NM_013433
- SwissProt
- O14787
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF VLTRLKSEDE61PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA SSLIRATIGI LITTIASKGE121LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL QKICEDSSEL LDSDALNRPL NIMIPKFLQF181FKHCSPKIRS HAIACVNQFI MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML241LEVRIDRLIP HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP301ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLPHEAE RPDGSEDAED361DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK GLLFHPEWVV KESGILVLGA421IAEGCMQGMV PYLPELIPHL IQCLSDKKAL VRSIACWTLS RYAHWVVSQP PDMHLKPLMT481ELLKRILDGN KRVQEAACSA FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD541AIGTLADSVG HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP601YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE GLGGHVEQLV661ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK PCIAEFMPIL GTNLNPEFIS721VCNNATWAIG EICMQMGAEM QPYVQMVLNN LVEIINRPNT PKTLLENTGR LTSPSAIPAI781TIGRLGYVCP QEVAPMLQQF IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI841FFCDAVASWV SPKDDLRDMF YKILHGFKDQ VGEDNWQQFS EQFPPLLKER LAAFYGVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
897 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.