Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TNNT2 / CTNT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122820-100
Catalog Number: LS-G122820
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cardiac Troponin T(TNNT2) (NM_001001431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cardiomyopathy | hypertrophic 2 | CMPD2 | CMD1D | CMH2 | Cardiac muscle troponin T | Cardiac Troponin T | LVNC6 | TNNT2 | TnTC | Troponin T type 2 (cardiac) | Troponin T | cardiac muscle | Troponin T2 | cardiac | CTnT | RCM3
- Origin Species
- Human
- NCBI GenBank
- NM_000364
- SwissProt
- P45379
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM61EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL121VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN181MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL241EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWKTRTRP LEQKLISEED301LAANDILDYK DDDDKV
316 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.