Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TNFRSF25 / DR3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106434-100
Catalog Number: LS-G106434
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DR3(TNFRSF25) (NM_148967), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APO-3 | APO3 | DDR3 | Death receptor 3 | DR3 | Death receptor beta | LARD | Protein WSL | TNFRSF25 | TR3 | WSL | WSL-1 | WSL-LR | Apoptosis inducing receptor | TNFRSF12 | WSL1 | Protein WSL-1 | TRAMP
- Origin Species
- Human
- NCBI GenBank
- NM_003790
- SwissProt
- Q93038
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQRPRGCAA VAAALLLVLL GARAQGGTRS PRCDCAGDFH KKIGLFCCRG CPAASQVALE61NCSAVADTRC GCKPGWFVEC QVSQCVSSSP FYCQPCLDCG ALHRHTRLLC SRRDTDCGTC121LPGFYEHGDG CVSCPTSTLG SCPERCAAVC GWRQMFWVQV LLAGLVVPLL LGATLTYTYR181HCWPHKPLVT ADEAGMEALT PPPATHLSPL DSAHTLLAPP DSSEKICTVQ LVGNSWTPGY241PETQEALCPQ VTWSWDQLPS RALGPAAAPT LSPESPAGSP AMMLQPGPQL YDVMDAVPAR301RWKEFVRTLG LREAEIEAVE VEIGRFRDQQ YEMLKRWRQQ QPAGLGAVYA ALERMGLDGC361VEDLRSRLQR GPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
372 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.