Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TNFRSF19 / TROY Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106443-100
Catalog Number: LS-G106443
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TROY(TNFRSF19) (NM_148957), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TAJ-alpha | TRADE | TROY | TNFRSF19 | Toxicity and JNK inducer | TAJ
- Origin Species
- Human
- NCBI GenBank
- NM_018647
- SwissProt
- Q9NS68
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALKVLLEQE KTFFTLLVLL GYLSCKVTCE SGDCRQQEFR DRSGNCVPCN QCGPGMELSK61ECGFGYGEDA QCVTCRLHRF KEDWGFQKCK PCLDCAVVNR FQKANCSATS DAICGDCLPG121FYRKTKLVGF QDMECVPCGD PPPPYEPHCA SKVNLVKIAS TASSPRDTAL AAVICSALAT181VLLALLILCV IYCKRQFMEK KPSWSLRSQD IQYNGSELSC FDRPQLHEYA HRACCQCRRD241SVQTCGPVRL LPSMCCEEAC SPNPATLGCG VHSAASLQAR NAGPAGEMVP TFFGSLTQSI301CGEFSDAWPL MQNPMGGDNI SFCDSYPELT GEDIHSLNPE LESSTSLDSN SSQDLVGGAV361PVQSHSENFT AATDLSRYNN TLVESASTQD ALTMRSQLDQ ESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
402 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.