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TMTC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMTC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104374-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMTC3 (NM_181783), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMTC3 | Protein SMILE | SMILE
Origin Species
Human
SwissProt
Q6ZXV5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MANINLKEIT LIVGVVTACY WNSLFCGFVF DDVSAILDNK DLHPSTPLKT LFQNDFWGTP
61MSEERSHKSY RPLTVLTFRL NYLLSELKPM SYHLLNMIFH AVVSVIFLKV CKLFLDNKSS
121VIASLLFAVH PIHTEAVTGV VGRAELLSSI FFLAAFLSYT RSKGPDNSII WTPIALTVFL
181VAVATLCKEQ GITVVGICCV YEVFIAQGYT LPLLCTTAGQ FLRGKGSIPF SMLQTLVKLI
241VLMFSTLLLV VIRVQVIQSQ LPVFTRFDNP AAVSPTPTRQ LTFNYLLPVN AWLLLNPSEL
301CCDWTMGTIP LIESLLDIRN LATFTFFCFL GMLGVFSIRY SGDSSKTVLM ALCLMALPFI
361PASNLFFPVG FVVAERVLYV PSMGFCILVA HGWQKISTKS VFKKLSWICL SMVILTHSLK
421TFHRNWDWES EYTLFMSALK VNKNNAKLWN NVGHALENEK NFERALKYFL QATHVQPDDI
481GAHMNVGRTY KNLNRTKEAE ESYMMAKSLM PQIIPGKKYA ARIAPNHLNV YINLANLIRA
541NESRLEEADQ LYRQAISMRP DFKQAYISRG ELLLKMNKPL KAKEAYLKAL ELDRNNADLW
601YNLAIVHIEL KEPNEALKNF NRALELNPKH KLALFNSAIV MQESGEVKLR PEARKRLLSY
661INEEPLDANG YFNLGMLAMD DKKDNEAEIW MKKAIKLQAD FRSALFNLAL LYSQTAKELK
721ALPILEELLR YYPDHIKGLI LKGDILMNQK KDILGAKKCF ERILEMDPSN VQGKHNLCVV
781YFEEKDLLKA ERCLLETLAL APHEEYIQRH LNIVRDKISS SSFIEPIFPT SKISSVEGKK
841IPTESVKEIR GESRQTQIVK TSDNKSQSKS NKQLGKNGDE ETPHKTTKDI KEIEKKRVAA
901LKRLEEIERI LNGE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

914 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.