Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMPRSS13 / MSP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119967-100
Catalog Number: LS-G119967
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMPRSS13 (NM_001077263), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MSP | Mosaic serine protease | MSPL | MSPS | TMPRSS11 | TMPRSS13
- Origin Species
- Human
- NCBI GenBank
- NM_001077263
- SwissProt
- Q9BYE2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MERDSHGNAS PARTPSAGAS PAQASPAGTP PGRASPAQAS PAQASPAGTP PGRASPAQAS61PAGTPPGRAS PGRASPAQAS PAQASPARAS PALASLSRSS SGRSSSARSA SVTTSPTRVY121LVRATPVGAV PIRSSPARSA PATRATRESP GTSLPKFTWR EGQKQLPLIG CVLLLIALVV181SLIILFQFWQ GHTGIRYKEQ RESCPKHAVR CDGVVDCKLK SDELGCVRFD WDKSLLKIYS241GSSHQWLPIC SSNWNDSYSE KTCQQLGFES AHRTTEVAHR DFANSFSILR YNSTIQESLH301RSECPSQRYI SLQCSHCGLR AMTGRIVGGA LASDSKWPWQ VSLHFGTTHI CGGTLIDAQW361VLTAAHCFFV TREKVLEGWK VYAGTSNLHQ LPEAASIAEI IINSNYTDEE DDYDIALMRL421SKPLTLSAHI HPACLPMHGQ TFSLNETCWI TGFGKTRETD DKTSPFLREV QVNLIDFKKC481NDYLVYDSYL TPRMMCAGDL RGGRDSCQGD SGGPLVCEQN NRWYLAGVTS WGTGCGQRNK541PGVYTKVTEV LPWIYSKMES EVRFRKSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
567 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.