Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMF1 / TMF-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114858-100
Catalog Number: LS-G114858
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TATA Element Modulatory Factor 1(TMF1) (NM_007114), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TMF | ARA160 | TATA element modulatory factor | TMF1
- Origin Species
- Human
- NCBI GenBank
- NM_007114
- SwissProt
- P82094
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 122.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSWFNASQLS SFAKQALSQA QKSIDRVLDI QEEEPSIWAE TIPYGEPGIS SPVSGGWDTS61TWGLKSNTEP QSPPIASPKA ITKPVRRTVV DESENFFSAF LSPTDVQTIQ KSPVVSKPPA121KSQRPEEEVK SSLHESLHIG QSRTPETTES QVKDSSLCVS GETLAAGTSS PKTEGKHEET181VNKESDMKVP TVSLKVSESV IDVKTTMESI SNTSTQSLTA ETKDIALEPK EQKHEDRQSN241TPSPPVSTFS SGTSTTSDIE VLDHESVISE SSASSRQETT DSKSSLHLMQ TSFQLLSASA301CPEYNRLDDF QKLTESCCSS DAFERIDSFS VQSLDSRSVS EINSDDELSG KGYALVPIIV361NSSTPKSKTV ESAEGKSEEV NETLVIPTEE AEMEESGRSA TPVNCEQPDI LVSSTPINEG421QTVLDKVAEQ CEPAESQPEA LSEKEDVCKT VEFLNEKLEK REAQLLSLSK EKALLEEAFD481NLKDEMFRVK EESSSISSLK DEFTQRIAEA EKKVQLACKE RDAAKKEIKN IKEELATRLN541SSETADLLKE KDEQIRGLME EGEKLSKQQL HNSNIIKKLR AKDKENENMV AKLNKKVKEL601EEELQHLKQV LDGKEEVEKQ HRENIKKLNS MVERQEKDLG RLQVDMDELE EKNRSIQAAL661DSAYKELTDL HKANAAKDSE AQEAALSREM KAKEELSAAL EKAQEEARQQ QETLAIQVGD721LRLALQRTEQ AAARKEDYLR HEIGELQQRL QEAENRNQEL SQSVSSTTRP LLRQIENLQA781TLGSQTSSWE KLEKNLSDRL GESQTLLAAA VERERAATEE LLANKIQMSS MESQNSLLRQ841ENSRFQAQLE SEKNRLCKLE DENNRYQVEL ENLKDEYVRT LEETRKEKTL LNSQLEMERM901KVEQERKKAI FTQETIKEKE RKPFSVSSTP TMSRSSSISG VDMAGLQTSF LSQDESHDHS961FGPMPISANG SNLYDAVRMG AGSSIIENLQ SQLKLREGEI THLQLEIGNL EKTRSIMAEE1021LVKLTNQNDE LEEKVKEIPK LRTQLRDLDQ RYNTILQMYG EKAEEAEELR LDLEDVKNMY1081KTQIDELLRQ SLSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1093 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.