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TMEM8A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM8A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM8A antibodies →
$504.00each
Catalog Number: LS-G110664-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM8A (NM_021259), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_021259
SwissProt
Q9HCN3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRAGTGTGG EAVAAVVAGP LLLLLLARPP PASAGYSGKS EVGLVSEHFS QAPQRLSFYS
61WYGSARLFRF RVPPDAVLLR WLLQVSRESG AACTDAEITV HFRSGAPPVI NPLGTSFPDD
121TAVQPSFQVG VPLSTAPRSN ASVNVSHPAP GDWFVAAHLP PSSQKIELKG LAPTCAYVFQ
181PELLVTRVVE ISIMEPDVPL PQTLLSHPSY LKVFVPDYTR ELLLELRDCV SNGSLGCPVR
241LTVGPVTLPS NFQKVLTCTG APWPCRLLLP SPPWDRWLQV TAESLVGPLG TVAFSAVAAL
301TACRPRSVTV QPLLQSSQNQ SFNASSGLLS PSPDHQDLGR SGRVDRSPFC LTNYPVTRED
361MDVVSVHFQP LDRVSVRVCS DTPSVMRLRL NTGMDSGGSL TISLRANKTE MRNETVVVAC
421VNAASPFLGF NTSLNCTTAF FQGYPLSLSA WSRRANLIIP YPETDNWYLS LQLMCPENAE
481DCEQAVVHVE TTLYLVPCLN DCGPYGQCLL LRRHSYLYAS CSCKAGWRGW SCTDNSTAQT
541VAQQRAATLL LTLSNLMFLA PIAVSVRRFF LVEASVYAYT MFFSTFYHAC DQPGEAVLCI
601LSYDTLQYCD FLGSGAAIWV TILCMARLKT VLKYVLFLLG TLVIAMSLQL DRRGMWNMLG
661PCLFAFVIMA SMWAYRCGHR RQCYPTSWQR WAFYLLPGVS MASVGIAIYT SMMTSDNYYY
721THSIWHILLA GSAALLLPPP DQPAEPWACS QKFPCHYQIC KNDREELYAV T
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

771 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.