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TMEM87B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM87B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM87B antibodies →
$725.00each
Catalog Number: LS-G108606-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM87B (NM_032824), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Transmembrane protein 87B | TMEM87B
Origin Species
Human
NCBI GenBank
NM_032824
SwissProt
Q96K49

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVAACRSVAG LLPRRRRCFP ARAPLLRVAL CLLCWTPAAV RAVPELGLWL ETVNDKSGPL
61IFRKTMFNST DIKLSVKSFH CSGPVKFTIV WHLKYHTCHN EHSNLEELFQ KHKLSVDEDF
121CHYLKNDNCW TTKNENLDCN SDSQVFPSLN NKELINIRNV SNQERSDVVA RTQKDGFHIF
181IVSIKTENTD ASWNLNVSLS MIGPHGYISA SDWPLMIFYM VMCIVYILYG ILWLTWSACY
241WKDILRIQFW IAAVIFLGML EKAVFYSEYQ NISNTGLSTQ GLLIFAELIS AIKRTLARLL
301VIIVSLGYGI VKPRLGTVMH RVIGLGLLYL IFAAVEGVMR VIGGSNHLAV VLDDIILAVI
361DSIFVWFIFI SLAQTMKTLR LRKNTVKFSL YRHFKNTLIF AVLASIVFMG WTTKTFRIAK
421CQSDWMERWV DDAFWSFLFS LILIVIMFLW RPSANNQRYA FMPLIDDSDD EIEEFMVTSE
481NLTEGIKLRA SKSVSNGTAK PATSENFDED LKWVEENIPS SFTDVALPVL VDSDEEIMTR
541SEMAEKMFSS EKIM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

554 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.