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TMEM87A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM87A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM87A antibodies →
$725.00each
Catalog Number: LS-G113183-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM87A (NM_015497), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_015497
SwissProt
Q8NBN3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAAWLQVL PVILLLLGAH PSPLSFFSAG PATVAAADRS KWHIPIPSGK NYFSFGKILF
61RNTTIFLKFD GEPCDLSLNI TWYLKSADCY NEIYNFKAEE VELYLEKLKE KRGLSGKYQT
121SSKLFQNCSE LFKTQTFSGD FMHRLPLLGE KQEAKENGTN LTFIGDKTAM HEPLQTWQDA
181PYIFIVHIGI SSSKESSKEN SLSNLFTMTV EVKGPYEYLT LEDYPLMIFF MVMCIVYVLF
241GVLWLAWSAC YWRDLLRIQF WIGAVIFLGM LEKAVFYAEF QNIRYKGESV QGALILAELL
301SAVKRSLART LVIIVSLGYG IVKPRLGVTL HKVVVAGALY LLFSGMEGVL RVTGAQTDLA
361SLAFIPLAFL DTALCWWIFI SLTQTMKLLK LRRNIVKLSL YRHFTNTLIL AVAASIVFII
421WTTMKFRIVT CQSDWRELWV DDAIWRLLFS MILFVIMVLW RPSANNQRFA FSPLSEEEEE
481DEQKEPMLKE SFEGMKMRST KQEPNGNSKV NKAQEDDLKW VEENVPSSVT DVALPALLDS
541DEERMITHFE RSKME
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

555 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.