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TMEM63A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM63A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM63A antibodies →
$504.00each
Catalog Number: LS-G113741-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM63A (NM_014698), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0489 | TMEM63A | Transmembrane protein 63A | KIAA0792
Origin Species
Human
NCBI GenBank
AL591895
SwissProt
O94886

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMDSPFLELW QSKAVSIREQ LGLGDRPNDS YCYNSAKNST VLQGVTFGGI PTVLLIDVSC
61FLFLILVFSI IRRRFWDYGR IALVSEADSE SRFQRLSSTS SSGQQDFENE LGCCPWLTAI
121FRLHDDQILE WCGEDAIHYL SFQRHIIFLL VVVSFLSLCV ILPVNLSGDL LDKDPYSFGR
181TTIANLQTDN DLLWLHTIFA VIYLFLTVGF MRHHTQSIKY KEENLVRRTL FITGLPRDAR
241KETVESHFRD AYPTCEVVDV QLCYNVAKLI YLCKEKKKTE KSLTYYTNLQ VKTGQRTLIN
301PKPCGQFCCC EVLGCEWEDA ISYYTRMKDR LLERITEEER HVQDQPLGMA FVTFQEKSMA
361TYILKDFNAC KCQSLQCKGE PQPSSHSREL YTSKWTVTFA ADPEDICWKN LSIQGLRWWL
421QWLGINFTLF LGLFFLTTPS IILSTMDKFN VTKPIHALNN PIISQFFPTL LLWSFSALLP
481SIVYYSTLLE SHWTKSGENQ IMMTKVYIFL IFMVLILPSL GLTSLDFFFR WLFDKTSSEA
541SIRLECVFLP DQGAFFVNYV IASAFIGNGM ELLRLPGLIL YTFRMIMAKT AADRRNVKQN
601QAFQYEFGAM YAWMLCVFTV IVAYSITCPI IAPFGLIYIL LKHMVDRHNL YFVYLPAKLE
661KGIHFAAVNQ ALAAPILCLF WLYFFSFLRL GMKAPATLFT FLVLLLTILV CLAHTCFGCF
721KHLSPLNYKT EEPASDKGSE AEAHMPPPFT PYVPRILNGL ASERTALSPQ QQQQQTYGAI
781HNISGTIPGQ CLAQSATGSV AAAPQEA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

807 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.