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TMEM62 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM62 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM62 antibodies →
$504.00each
Catalog Number: LS-G109645-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM62 (NM_024956), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMEM62 | Transmembrane protein 62
Origin Species
Human
NCBI GenBank
NM_024956
SwissProt
Q0P6H9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAVLALRVV AGLAAAALVA MLLEHYGLAG QPSPLPRPAP PRRPHPAPGP GDSNIFWGLQ
61ISDIHLSRFR DPGRAVDLEK FCSETIDIIQ PALVLATGDL TDAKTKEQLG SRQHEVEWQT
121YQGILKKTRV MEKTKWLDIK GNHDAFNIPS LDSIKNYYRK YSAVRRDGSF HYVHSTPFGN
181YSFICVDATV NPGPKRPYNF FGILDKKKME ELLLLAKESS RSNHTIWFGH FTTSTILSPS
241PGIRSIMSSA IAYLCGHLHT LGGLMPVLHT RHFQGTLELE VGDWKDNRRY RIFAFDHDLF
301SFADLIFGKW PVVLITNPKS LLYSCGEHEP LERLLHSTHI RVLAFSLSSI TSVTVKIDGV
361HLGQAVHVSG PIFVLKWNPR NYSSGTHNIE VIVQDSAGRS KSVHHIFSVQ ENNHLSFDPL
421ASFILRTDHY IMARVLFVLI VLSQLTILII FRYRGYPELK EPSGFINLTS FSLHVLSKIN
481IFYYSVLLLT LYTVLGPWFF GEIIDGKFGC CFSFGIFVNG HFLQGSITFI IGILQLAFFN
541IPLMAYMCWS LLQRCFGHNF RSHLHQRKYL KIMPVHLLML LLYIWQVYSC YFLYATYGTL
601AFLFSPLRTW LTLLTPVLIR YVWTLNSTKF GIFMVQLKSH LSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

643 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.