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TMEM59 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM59 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM59 antibodies →
$504.00each
Catalog Number: LS-G116352-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM59 (NM_004872), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C1orf8 | HSPC001 | Liver membrane-bound protein | TMEM59 | Transmembrane protein 59
Origin Species
Human
NCBI GenBank
NM_004872
SwissProt
Q9BXS4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
36 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAPKGSLWV RTQLGLPPLL LLTMALAGGS GTASAEAFDS VLGDTASCHR ACQLTYPLHT
61YPKEEELYAC QRGCRLFSIC QFVDDGIDLN RTKLECESAC TEAYSQSDEQ YACHLGCQNQ
121LPFAELRQEQ LMSLMPKMHL LFPLTLVRSF WSDMMDSAQS FITSSWTFYL QADDGKIVIF
181QSKPEIQYAP HLEQEPTNLR ESSLSKMSSD LQMRNSQAHR NFLEDGESDG FLRCLSLNSG
241WILTTTLVLS VMVLLWICCA TVATAVEQYV PSEKLSIYGD LEFMNEQKLN RYPASSLVVV
301RSKTEDHEEA GPLPTKVNLA HSEI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

324 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.