Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMEM259 / Membralin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120887-100
Catalog Number: LS-G120887
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Membralin(TMEM259) (NM_001033026), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASBABP1 | C19orf6 | MBRL | Membralin | R32184_3
- Origin Species
- Human
- NCBI GenBank
- NM_001033026
- SwissProt
- Q4ZIN3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEHVEPAAP GPGPNGGGGG PAPARGPRTP NLNPNPLINV RDRLFHALFF KMAVTYSRLF61PPAFRRLFEF FVLLKALFVL FVLAYIHIVF SRSPINCLEH VRDKWPREGI LRVEVRHNSS121RAPVFLQFCD SGGRGSFPGL AVEPGSNLDM EDEEEEELTM EMFGNSSIKF ELDIEPKVFK181PPSSTEALND SQEFPFPETP TKVWPQDEYI VEYSLEYGFL RLSQATRQRL SIPVMVVTLD241PTRDQCFGDR FSRLLLDEFL GYDDILMSSV KGLAENEENK GFLRNVVSGE HYRFVSMWMA301RTSYLAAFAI MVIFTLSVSM LLRYSHHQIF VFIVDLLQML EMNMAIAFPA APLLTVILAL361VGMEAIMSEF FNDTTTAFYI ILIVWLADQY DAICCHTSTS KRHWLRFFYL YHFAFYAYHY421RFNGQYSSLA LVTSWLFIQH SMIYFFHHYE LPAILQQVRI QEMLLQAPPL GPGTPTALPD481DMNNNSGAPA TAPDSAGQPP ALGPVSPGAS GSPGPVAAAP SSLVAAAASV AAAAGGDLGW541MAETAAIITD ASFLSGLSAS LLERRPASPL GPAGGLPHAP QDSVPPSDSA ASDTTPLGAA601VGGPSPASMA PTEAPSEVGSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
620 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.