Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMEM248 / c7orf42 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112062-100
Catalog Number: LS-G112062
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C7orf42(TMEM248) (NM_017994), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C7orf42 | Transmembrane protein 248 | UPF0458 protein C7orf42 | TMEM248
- Origin Species
- Human
- NCBI GenBank
- NM_017994
- SwissProt
- Q9NWD8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFSINPLENL KVYISSRPPL VVFMISVSAM AIAFLTLGYF FKIKEIKSPE MAEDWNTFLL61RFNDLDLCVS ENETLKHLTN DTTTPESTMT SGQARASTQS PQALEDSGPV NISVSITLTL121DPLKPFGGYS RNVTHLYSTI LGHQIGLSGR EAHEEINITF TLPTAWSSDD CALHGHCEQV181VFTACMTLTA SPGVFPVTVQ PPHCVPDTYS NATLWYKIFT TARDANTKYA QDYNPFWCYK241GAIGKVYHAL NPKLTVIVPD DDRSLINLHL MHTSYFLFVM VITMFCYAVI KGRPSKLRQS301NPEFCPEKVA LAEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
314 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.