Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMEM237 / ALS2CR4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106279-100
Catalog Number: LS-G106279
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALS2CR4(TMEM237) (NM_152388), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ALS2CR4 | JBTS14 | TMEM237 | Transmembrane protein 237
- Origin Species
- Human
- SwissProt
- Q96Q45
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGKNPVRPPR AIPPVPSQDD IPLSRPKKKK PRTKNTPASA SLEGLAQTAG RRPSEGNEPS61TKELKEHPEA PVQRRQKKTR LPLELETSST QKKSSSSSLL RNENGIDAEQ AEEAVIQKPR121RKTKKTQPAE LQYANELGVE DEDIITDEQT TVEQQSVFTA PTGISQPVGK VFVEKSRRFQ181AADRSELIKT TENIDVSMDV KPSWTTIDVA LTVHRAFRMI GLFSHGFLAG CAVWNIVVIY241VLAGDQLSNL SNILQQYKTL AYPFQSLLYL LLALSTISAF DRIDFAKISV AIRNFLALDP301TALASFLYFT ALILSLSQQM TSDRIHLYTP SSVNGSLWEA GIEEQILQPW IVVNLVVALL361VGLSWLFLSY RPGMDLSEEL MFSSEVEEYP DKEKEIKASSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
400 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.