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TMEM209 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM209 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM209 antibodies →
$504.00each
Catalog Number: LS-G108522-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM209 (NM_032842), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Transmembrane protein 209 | NET31 | TMEM209
Origin Species
Human
NCBI GenBank
NM_032842
SwissProt
Q96SK2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMQGEAHPSA SLIDRTIKMR KETEARKVVL AWGLLNVSMA GMIYTEMTGK LISSYYNVTY
61WPLWYIELAL ASLFSLNALF DFWRYFKYTV APTSLVVSPG QQTLLGLKTA VVQTTPPHDL
121AATQIPPAPP SPSIQGQSVL SYSPSRSPST SPKFTTSCMT GYSPQLQGLS SGGSGSYSPG
181VTYSPVSGYN KLASFSPSPP SPYPTTVGPV ESSGLRSRYR SSPTVYNSPT DKEDYMTDLR
241TLDTFLRSEE EKQHRVKLGS PDSTSPSSSP TFWNYSRSMG DYAQTLKKFQ YQLACRSQAP
301CANKDEADLS SKQAAEEVWA RVAMNRQLLD HMDSWTAKFR NWINETILVP LVQEIESVST
361QMRRMGCPEL QIGEASITSL KQAALVKAPL IPTLNTIVQY LDLTPNQEYL FERIKELSQG
421GCMSSFRWNR GGDFKGRKWD TDLPTDSAII MHVFCTYLDS RLPPHPKYPD GKTFTSQHFV
481QTPNKPDVTN ENVFCIYQSA INPPHYELIY QRHVYNLPKG RNNMFHTLLM FLYIIKTKES
541GMLGRVNLGL SGVNILWIFG E
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

561 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.