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TMEM183B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM183B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM183B antibodies →
$504.00each
Catalog Number: LS-G120115-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM183B (NM_001079809), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMEM183B | Transmembrane protein 183B | C1orf37-DUP
Origin Species
Human
NCBI GenBank
NM_001079809
SwissProt
Q1AE95

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARGPGPLGR PRPDTVAMPK RGKRLKFRAH DACSGRVTVA DYANSDPAVV RSGRVKKAVA
61NAVQQEVKSL CGLEASQVPA EEALSGAGEP CDIIDSSDEM DAQEESIHER TVSRKKKSKR
121HKEELDGAGG EEYPMDIWLL LASYIRPEDI VNFSLICKNA WTVTCTAAFW TRLYRRHYTL
181DASLPLRLRP ESMEKLRCLR ACVIRSLYHM YEPFAARISK NPAIPESTPS TLKNSKCLLF
241WCRKIVGNRQ EPMWEFNFKF KKQSPRLKSK CTGGLQPPVQ YEDVHTNPDQ DCCLLQVTTL
301NFIFIPIVMG MIFTLFTINV STDMRHHRVR LVFQDSPVHG GRKLRSEQGV QVILDPVHSV
361RLFDWWHPQY PFSLRA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

376 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.