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TMEM180 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM180 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM180 antibodies →
$725.00each
Catalog Number: LS-G109739-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM180(MFSD13A) (NM_024789), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA18I14.8 | C10orf77 | RP11-18I14.8 | TMEM180 | Transmembrane protein 180
Origin Species
Human
NCBI GenBank
AK026182
SwissProt
Q14CX5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGLGQPQAWL LGLPTAVVYG SLALFTTILH NVFLLYYVDT FVSVYKINKM AFWVGETVFL
61LWNSLNDPLF GWLSDRQFLS SQPRSGAGLS SRAVVLARVQ ALGWHGPLLA LSFLAFWVPW
121APAGLQFLLC LCLYDGFLTL VDLHHHALLA DLALSAHDRT HLNFYCSLFS AAGSLSVFAS
181YAFWNKEDFS SFRAFCVTLA VSSGLGFLGA TQLLRRRVEA ARKDPGCSGL VVDSGLCGEE
241LLVGSEEADS ITLGRYLRQL ARHRNFLWFV SMDLVQVFHC HFNSNFFPLF LEHLLSDHIS
301LSTGSILLGL SYVAPHLNNL YFLSLCRRWG VYAVVRGLFL LKLGLSLLML LAGPDHLSLL
361CLFIASNRVF TEGTCKLLTL VVTDLVDEDL VLNHRKQAAS ALLFGMVALV TKPGQTFAPL
421LGTWLLCFYT GHDLFQQSLI TPVGSAHPWP EPPAPAPAQA PTLRQGCFYL LVLVPITCAL
481LQLFTWSQFT LHGRRLHMVK AQRQNLSQAQ TLDVKMV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

517 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.