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TMEM168 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM168 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM168 antibodies →
$725.00each
Catalog Number: LS-G110339-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM168 (NM_022484), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Transmembrane protein 168 | TMEM168
Origin Species
Human
NCBI GenBank
NM_022484
SwissProt
Q9H0V1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCKSLRYCFS HCLYLAMTRL EEVNREVNMH SSVRYLGYLA RINLLVAICL GLYVRWEKTA
61NSLILVIFIL GLFVLGIASI LYYYFSMEAA SLSLSNLWFG FLLGLLCFLD NSSFKNDVKE
121ESTKYLLLTS IVLRILCSLV ERISGYVRHR PTLLTTVEFL ELVGFAIAST TMLVEKSLSV
181ILLVVALAML IIDLRMKSFL AIPNLVIFAV LLFFSSLETP KNPIAFACFF ICLITDPFLD
241IYFSGLSVTE RWKPFLYRGR ICRRLSVVFA GMIELTFFIL SAFKLRDTHL WYFVIPGFSI
301FGIFWMICHI IFLLTLWGFH TKLNDCHKVY FTHRTDYNSL DRIMASKGMR HFCLISEQLV
361FFSLLATAIL GAVSWQPTNG IFLSMFLIVL PLESMAHGLF HELGNCLGGT SVGYAIVIPT
421NFCSPDGQPT LLPPEHVQEL NLRSTGMLNA IQRFFAYHMI ETYGCDYSTS GLSFDTLHSK
481LKAFLELRTV DGPRHDTYIL YYSGHTHGTG EWALAGGDTL RLDTLIEWWR EKNGSFCSRL
541IIVLDSENST PWVKEVRKIN DQYIAVQGAE LIKTVDIEEA DPPQLGDFTK DWVEYNCNSS
601NNICWTEKGR TVKAVYGVSK RWSDYTLHLP TGSDVAKHWM LHFPRITYPL VHLANWLCGL
661NLFWICKTCF RCLKRLKMSW FLPTVLDTGQ GFKLVKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

697 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.