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TMEM161A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM161A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM161A antibodies →
$504.00each
Catalog Number: LS-G112198-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM161A (NM_017814), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMEM161A | AROS-29 | Transmembrane protein 161A
Origin Species
Human
NCBI GenBank
NM_017814
SwissProt
Q9NX61

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVLGVQLVV TLLTATLMHR LAPHCSFARW LLCNGSLFRY KHPSEEELRA LAGKPRPRGR
61KERWANGLSE EKPLSVPRDA PFQLETCPLT TVDALVLRFF LEYQWFVDFA VYSGGVYLFT
121EAYYYMLGPA KETNIAVFWC LLTVTFSIKM FLTVTRLYFS AEEGGERSVC LTFAFLFLLL
181AMLVQVVREE TLELGLEPGL ASMTQNLEPL LKKQGWDWAL PVAKLAIRVG LAVVGSVLGA
241FLTFPGLRLA QTHRDALTMS EDRPMLQFLL HTSFLSPLFI LWLWTKPIAR DFLHQPPFGE
301TRFSLLSDSA FDSGRLWLLV VLCLLRLAVT RPHLQAYLCL AKARVEQLRR EAGRIEAREI
361QQRVVRVYCY VTVVSLQYLT PLILTLNCTL LLKTLGGYSW GLGPAPLLSP DPSSASAAPI
421GSGEDEVQQT AARIAGALGG LLTPLFLRGV LAYLIWWTAA CQLLASLFGL YFHQHLAGS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.