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TMEM151B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM151B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM151B antibodies →
$504.00each
Catalog Number: LS-G125780-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM151B (NM_001137560), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA444E17.5 | C6orf137 | TMEM151B | Transmembrane protein 151B | Transmembrane protein 193 | TMEM193
Origin Species
Human
NCBI GenBank
NM_001137560
SwissProt
Q8IW70

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSPPGSAAGE SAAGGGGGGG GPGVSEELTA AAAAAAADEG PAREEQRPIQ PSFTKSLCRE
61SHWKCLLLSL LMYGCLGAVA WCHVTTVTRL TFSSAYQGNS LMYHDSPCSN GYVYIPLAFL
121LMLYAVYLVE CWHCQARHEL QHRVDVSSVR ERVGRMQQAT PCIWWKAISY HYVRRTRQVT
181RYRNGDAYTT TQVYHERVNT HVAEAEFDYA RCGVRDVSKT LVGLEGAPAT RLRFTKCFSF
241ASVEAENAYL CQRARFFAEN EGLDDYMEAR EGMHLKNVDF REFMVAFPDP ARPPWYACSS
301AFWAAALLTL SWPLRVLAEY RTAYAHYHVE KLFGLEGPGS ASSAGGGLSP SDELLPPLTH
361RLPRVNTVDS TELEWHIRSN QQLVPSYSEA VLMDLAGLGT RCGGAGGGYA PSCRYGGVGG
421PGAAGVAPYR RSCEHCQRAV SSSSIFSRSA LSICASPRAG PGPGGGAGCG GSRFSLGRLY
481GSRRSCLWRS RSGSVNEASC PTEQTRLSSQ ASMGDDEDDD EEEAGPPPPY HDALYFPVLI
541VHRQEGCLGH SHRPLHRHGS CVETSL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

566 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.