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TMEM146 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM146 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CATSPERD antibodies →
$504.00each
Catalog Number: LS-G106005-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM146(CATSPERD) (NM_152784), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CatSperdelta | Transmembrane protein 146 | TMEM146 | CatSper-delta | CATSPERD
Origin Species
Human
SwissProt
Q86XM0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLVAAVTMWL RPLVTAQLCR SRTVRTGKVF NLIQDVQGDR LYFHPTTTRL IKHPCEKNIA
61LYLGKQVFFT MDNFETSLLP FTIPTSMQVG VPEVTSAHFA GSLLLLVVDQ KVYIYDYENN
121SWSMSLGIKH PVTHVSGDNC CYTGSLFCVH VSNLVFAYFR GDQISQTYIY YSNTGGFSFW
181KYHYDRQAEI IGSLGGIFHF FSLSQVAMLV VNQGKGMFKY SDHPLNRSFG LSFDYNGTLD
241ILIAPGQRGI LLLWFENSLL FSHNAGQLVD TVRVKKGDQT LFSSIFEAKI TIHNIAVTEN
301ELAVITREDN LYYGNLGIVP SSIIKFADQY IWSEDVALMF RSPGTLEILT PLRDTAFPAF
361DFQKCLVNIQ ALLMDPELHV GKCKIEFLTG EFIYRMYTID MHSQLELTAS LIPQPGTSLI
421PLVMVSNPHS LGFQATFYEN GYTSDGNTKY KLDIFLKQQQ HWGRTDSNFT SSLKKATMST
481LTVDIANKEI SCVDIKPLSA LISVGCDLDK KIVIQNKVSA CSMGILDPLT LQDNYSFIIE
541KEFYDPGFQG QQSSEDLHVF YSYQQLGCPL LVYYDTLWKP VVELWRKDSF QEVIDAEYVL
601LEVNGQFSYS YSLTAQSAMC TSQPQNWTTM IKEFGGPFFW NRENYVSCHD PNNNAPLRWP
661DVQYQILGGR TANQIIFGHN GFYVFYISIV DPYYSYCQLE TIFSIYVYGA FPVQLVSAGV
721VILLIISSIL GSVWLAYKTP KLLRTARGRR IKKCATQLCR RCKTVCQFRA SATARAGTEP
781PGRHRTPHGG RSDH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

794 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.