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TMEM132E Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM132E Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM132E antibodies →
$725.00each
Catalog Number: LS-G102873-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM132E (NM_207313), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMEM132E | Transmembrane protein 132E
Origin Species
Human
NCBI GenBank
NM_207313
SwissProt
Q6IEE7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
106.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPGMSGRGG AALLCLSALL AHASGRSHPA SPSPPGPQAS PVLPVSYRLS HTRLAFFLRE
61ARPPSPAVAN SSLQRSEPFV VFQTKELPVL NVSLGPFSTS QVVARELLQP SSTLDIPERL
121TVNWKVRAFI VRSHVPASQP VVQVLFYVAG RDWDDFGVTE RLPCVRLHAF RDAREHPLLR
181IGSISLFRPP PRRTLQEHRL DSNLMIRLPD RPLKPGEVLS ILLYLAPNSS SPSSPSVEHF
241TLRVKAKKGV TLLGTKSRSG QWHVTSELLT GAKHSTATVD VAWAQSTPLP PREGQGPLEI
301LQLDFEMENF TSQSVKRRIM WHIDYRGHGA LPDLERAVTE LTVIQRDVQA ILPLAMDTEI
361INTAILTGRT VAIPVKVIAI EVNGLVLDIS ALVECESDNE DIIKVSSSCD YVFVSGKESR
421GSMNARVTFR YDVLNAPLEM TVWVPKLPLH IELSDARLSQ VKGWRVPILP DRRSVRESED
481EDEEEEERRQ SASRGCTLQY QHATLQVFTQ FHTTSSEGTD QVVTMLGPDW LVEVTDLVSD
541FMRVGDPRVA HMVDSSTLAG LEPGTTPFKV VSPLTEAVLG ETLLTVTEEK VSITQLQAQV
601VASLALSLRP SPGSSHTILA TTAAQQTLSF LKQEALLSLW LSYSDGTTAP LSLYSPRDYG
661LLVSSLDEHV ATVTQDRAFP LVVAEAEGSG ELLRAELTIA ESCQKTKRKS VLATTPVGLR
721VHFGRDEEDP TYDYPGPSQP GPGGGEDEAR GAGPPGSALP APEAPGPGTA SPVVPPTEDF
781LPLPTGFLQV PRGLTDLEIG MYALLGVFCL AILVFLINCI VFVLRYRHKR IPPEGQTSMD
841HSHHWVFLGN GQPLRVQGEL SPPAGNPLET VPAFCHGDHH SSGSSQTSVQ SQVHGRGDGS
901SGGSARDQAE DPASSPTSKR KRVKFTTFTT LPSEELAYDS VPAGEEDEEE EEDLGWGCPD
961VAGPTRPTAP PDLHNYMRRI KEIA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

984 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.