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TMEM132C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM132C Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM132C antibodies →
$725.00each
Catalog Number: LS-G125639-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM132C (NM_001136103), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMEM132C | Transmembrane protein 132C
Origin Species
Human
NCBI GenBank
NM_001136103
SwissProt
Q8N3T6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.65 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLTSNFLGPT NKFSFDWKLK AHILRDKVYL SRPKVQVLFH IMGRDWDDHG AGEKLPCLRV
61FAFRETREVR GSCRLKGDLG LCVAELELLS SWFSAPTVGA GRKKSMDQPE GTPVELYYTV
121HPGNERGDCA GGDFRKGNAI RPGKDGLEET TSHLQRIGTV GLYRAQDSAQ LSELRLDGNV
181VIWLPSRPVK QGEVVTAYVT ISSNSSVDLF ILRAKVKKGV NILSAQTREP RQWGVKQEVG
241SGGKHVTATV ACQRLGPSPR NRSSSLFNEV VQMNFEIASF SSLSGTQPIT WQVEYPRKGT
301TDIAVSEIFV SQKDLVGIVP LAMDTEILNT AVLTGKTVAM PIKVVSVEEN SAVMDISESV
361ECKSTDEDVI KVSERCDYIF VNGKEIKGKM DAVVNFTYQY LSAPLCVTVW VPRLPLQIEV
421SDTELSQIKG WRVPIVTNKR PTRESEDEDE EERRGRGCAL QYQHATVRVL TQFVSEGAGP
481WGQPNYLLSP NWQFDITHLV ADFMKLEEPH VATLQDSRVL VGREVGMTTI QVLSPLSDSI
541LAEKTITVLD DKVSVTDLAI QLVAGLSVAL YPNAENSKAV TAVVTAEEVL RTPKQEAVFS
601TWLQFSDGSV TPLDIYDTKD FSLAATSQDE AVVSVPQPRS PRWPVVVAEG EGQGPLIRVD
661MTIAEACQKS KRKSILAVGV GNVRVKFGQN DADSSPGGDY EEDEIKNHAS DRRQKGQHHE
721RTGQDGHLYG SSPVEREEGA LRRATTTARS LLDNKVVKNS RADGGRLAGE GQLQNIPIDF
781TNFPAHVDLP KAGSGLEEND LVQTPRGLSD LEIGMYALLG VFCLAILVFL INCATFALKY
841RHKQVPLEGQ ASMTHSHDWV WLGNEAELLE SMGDAPPPQD EHTTIIDRGP GACEESNHLL
901LNGGSHKHVQ SQIHRSADSG GRQGREQKQD PLHSPTSKRK KVKFTTFTTI PPDDSCPTVN
961SIVSSNDEDI KWVCQDVAVG APKELRNYLE KLKDKA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

996 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.