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TMEM132B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM132B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM132B antibodies →
$725.00each
Catalog Number: LS-G108107-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM132B (NM_052907), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1786 | TMEM132B | Transmembrane protein 132B | KIAA1906
Origin Species
Human
SwissProt
Q14DG7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
119.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFGAASRMDT TAVCTGGVTE SRGIVDSLQK FSSLPAYLPT NLHISNAEES FFLKEANQDL
61TRNSSLQARV EPFFIYRART PPIINASYGP FSVEKIIPQE LLLTSTAFGN MDKFPFNWKL
121KSHILDSSIY SNRPKVQTLF YVTGMGWDDS DLTEDLPCVK MFAFPEAREV AASCRLQGAP
181GLCVAELELL PEWFSSGLDL EPEEEIPALL GGTTMELFFT LYPADKAGQC PLEEEGKWEN
241NIHSGLESPQ QAFPARERIG SVVVYPTQDD LKWSLVSLDE NVVISVPLNL VREGDTATFL
301VSLTSSSVAD QFTLRIKAAA GVKITAVRVS SEDQWAVQEE IDNGSTQTSA TLTCMGHRPD
361TQSRVNGSFY EILQVDFGID NSSDLAGAQQ ITWQVEYPIE DSMSELVVSE IFVSQTTFVG
421IVPLAMDTEV LNTAILTGKP VSVPVKVVGV QEDGSVVDVS ESVECKSADE DVIKVSNNCD
481SIFVNGKEMK SKVDTIVNFT HQHFTSQFEV TVWAPRLPLQ IEISDTELSQ IKGWRIPVAA
541NRRPTRESDD EDDEEKKGRG CSLQYQHATV RVLTQFVAES PDLGQLTYML GPDWQFDITD
601LVTEFMKVEE PKIAQLQDGR TLAGREPGIT TVQVLSPLSD SILAEKTVIV LDDRVTIAEL
661GVQLVAGMSL SLQPHRADKR AIVSTAAALD VLQSPQQEAI VSSWILFSDG SVTPLDIYDP
721KDYSVTVSSL DEMVVSVQAN LESKWPIVVA EGEGQGPLIK LEMMISEPCQ KTKRKSVLAV
781GKGNVKVKFE PSSDEHQGGS NDIEGINREY KDHLSNSIER EGNQERAVQE WFHRGTPVGQ
841EESTNKSTTP QSPMEGKNKL LKSGGPDAFT SFPTQGKSPD PNNPSDLTVT SRGLTDLEIG
901MYALLCVFCL AILVFLINCV AFAWKYRHKR FAVSEQGNIP HSHDWVWLGN EVELLENPVD
961ITLPSEECTT MIDRGLQFEE RNFLLNGSSQ KTFHSQLLRP SDYVYEKEIK NEPMNSSGPK
1021RKRVKFTSYT TILPEDGGPY TNSILFDSDD NIKWVCQDMG LGDSQDFRDY MESLQDQM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1078 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.