Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMEM132A / HSPA5BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112166-100
Catalog Number: LS-G112166
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM132A (NM_017870), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSPA5BP1 | KIAA1583 | GBP | HSPA5-binding protein 1 | TMEM132A | GRP78-binding protein | Transmembrane protein 132A
- Origin Species
- Human
- NCBI GenBank
- NM_017870
- SwissProt
- Q24JP5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 110 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCARMAGRTT AAPRGPYGPW LCLLVALALD VVRVDCGQAP LDPVYLPAAL ELLDAPEHFR61VQQVGHYPPA NSSLSSRSET FLLLQPWPRA QPLLRASYPP FATQQVVPPR VTEPHQRPVP121WDVRAVSVEA AVTPAEPYAR VLFHLKGQDW PPGSGSLPCA RLHATHPAGT AHQACRFQPS181LGACVVELEL PSHWFSQAST TRAELAYTLE PAAEGPGGCG SGEENDPGEQ ALPVGGVELR241PADPPQYQEV PLDEAVTLRV PDMPVRPGQL FSATLLLRHN FTASLLTLRI KVKKGLHVTA301ARPAQPTLWT AKLDRFKGSR HHTTLITCHR AGLTEPDSSS PLELSEFLWV DFVVENSTGG361GVAVTRPVTW QLEYPGQAPE AEKDKMVWEI LVSERDIRAL IPLAKAEELV NTAPLTGVPQ421HVPVRLVTVD GGGALVEVTE HVGCESANTQ VLQVSEACDA VFVAGKESRG ARGVRVDFWW481RRLRASLRLT VWAPLLPLRI ELTDTTLEQV RGWRVPGPAE GPAEPAAEAS DEAERRARGC541HLQYQRAGVR FLAPFAAHPL DGGRRLTHLL GPDWLLDVSH LVAPHARVLD SRVASLEGGR601VVVGREPGVT SIEVRSPLSD SILGEQALAV TDDKVSVLEL RVQPVMGISL TLSRGTAHPG661EVTATCWAQS ALPAPKQEVA LSLWLSFSDH TVAPAELYDR RDLGLSVSAE EPGAILPAEE721QGAQLGVVVS GAGAEGLPLH VALHPPEPCR RGRHRVPLAS GTAWLGLPPA STPAPALPSS781PAWSPPATEA TMGGKRQVAG SVGGNTGVRG KFERAEEEAR KEETEAREEE EEEEEEMVPA841PQHVTELELG MYALLGVFCV AIFIFLVNGV VFVLRYQRKE PPDSATDPTS PQPHNWVWLG901TDQEELSRQL DRQSPGPPKG EGSCPCESGG GGEAPTLAPG PPGGTTSSSS TLARKEAGGR961RKRVEFVTFA PAPPAQSPEE PVGAPAVQSI LVAGEEDIRW VCEDMGLKDP EELRNYMERI1021RGSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1024 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.