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TMEM117 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM117 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM117 antibodies →
$504.00each
Catalog Number: LS-G108962-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM117 (NM_032256), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMEM117 | Transmembrane protein 117
Origin Species
Human
NCBI GenBank
NM_032256
SwissProt
Q9H0C3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1MGKDFRYYFQ HPWSRMIVAY LVIFFNFLIF AEDPVSHSQT EANVIVVGNC FSFVTNKYPR
61GVGWRILKVL LWLLAILTGL IAGKFLFHQR LFGQLLRLKM FREDHGSWMT MFFSTILFLF
121IFSHIYNTIL LMDGNMGAYI ITDYMGIRNE SFMKLAAVGT WMGDFVTAWM VTDMMLQDKP
181YPDWGKSARA FWKKGNVRIT LFWTVLFTLT SVVVLVITTD WISWDKLNRG FLPSDEVSRA
241FLASFILVFD LLIVMQDWEF PHFMGDVDVN LPGLHTPHMQ FKIPFFQKIF KEEYRIHITG
301KWFNYGIIFL VLILDLNMWK NQIFYKPHEY GQYIGPGQKI YTVKDSESLK DLNRTKLSWE
361WRSNHTNPRT NKTYVEGDMF LHSRFIGASL DVKCLAFVPS LIAFVWFGFF IWFFGRFLKN
421EPRMENQDKT YTRMKRKSPS EHSKDMGITR ENTQASVEDP LNDPSLVCIR SDFNEIVYKS
481SHLTSENLSS QLNESTSATE ADQDPTTSKS TPTN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.