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TMEM104 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM104 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM104 antibodies →
$504.00each
Catalog Number: LS-G101495-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM104 (NM_017728), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMEM104 | Transmembrane protein 104
Origin Species
Human
NCBI GenBank
NM_017728
SwissProt
Q8NE00

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGEITETGE LYSSYVGLVY MFNLIVGTGA LTMPKAFATA GWLVSLVLLV FLGFMSFMTT
61TFVIEAMAAA NAQLHWKRME NLKEEEDDDS STASDSDVLI RDNYERAEKR PILSVQRRGS
121PNPFEITDRV EMGQMASMFF NKVGVNLFYF CIIVYLYGDL AIYAAAVPFS LMQVTCSATG
181NDSCGVEADT KYNDTDRCWG PLRRVDAYRI YLAIFTLLLG PFTFFDVQKT KYLQILTSLM
241RWIAFAVMIV LALIRIGHGQ GEGHPPLADF SGVRNLFGVC VYSFMCQHSL PSLITPVSSK
301RHLTRLVFLD YVLILAFYGL LSFTAIFCFR GDSLMDMYTL NFARCDVVGL AAVRFFLGLF
361PVFTISTNFP IIAVTLRNNW KTLFHREGGT YPWVVDRVVF PTITLVPPVL VAFCTHDLES
421LVGITGAYAG TGIQYVIPAF LVYHCRRDTQ LAFGCGVSNK HRSPFRHTFW VGFVLLWAFS
481CFIFVTANII LSETKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.