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TMEM102 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMEM102 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMEM102 antibodies →
$504.00each
Catalog Number: LS-G104649-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM102 (NM_178518), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CBAP | TMEM102 | Transmembrane protein 102
Origin Species
Human
SwissProt
Q3UPR7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54 kda
Protein Type
Lysate
Amino Acid Sequence
1MASAVWGSAP WWGPPPPAPA RPLTDIDFCS GAQLQELTQL IQELGVQESW SDGPKPGADL
61LRAKDFVFSL LGLVHRRDPR FPPQAELLLL RGGIREGSLD LGHAPLGPYA RGPHYDAGFT
121LLVPMFSLDG TELQLDLESC YAQVCLPEMV CGTPIREMWQ DCLGPPVPGA RDSIHRTESE
181ESSKDWQSSV DQPHSYVTEH EAPVSLEKSP SDVSASESPQ HDVVDLGSTA PLKTMSSDVT
241KAAVESPVPK PSEAREAWPT LCSAQVAAWF FATLAAVAES LIPVPGAPRL VHAARHAGFT
301TVLLATPEPP RRLLLFDLIP VVSVAGWPEG ARSHSWAGPL ASESASFYLV PGGGTERPCA
361SAWQLCFARQ ELALKARIPA PLLQAHAAAQ ALLRPLVAGT RAAAPYLLRT LLYWACERLP
421ALYLARPENA GACCLGLLDE LGRVLEAGTL PHYFLNGRQL RTGDDSAALL GELARLRGDP
481ARALRAAVEE AKVARKGGGL AGVGGGAH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.