Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMEM1 / TRAPPC10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117453-100
Catalog Number: LS-G117453
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM1(TRAPPC10) (NM_003274), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GT334 | TRAPP subunit TMEM1 | Protein GT334 | TRAPPC10 | EHOC-1 | EHOC1 | TMEM1 | Transmembrane protein 1 | TRAPP 130 kDa subunit | TRS130 | TRS30
- Origin Species
- Human
- NCBI GenBank
- NM_003274
- SwissProt
- P48553
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 142 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDASEEPLPP VIYTMENKPI VTCAGDQNLF TSVYPTLSQQ LPREPMEWRR SYGRAPKMIH61LESNFVQFKE ELLPKEGNKA LLTFPFLHIY WTECCDTEVY KATVKDDLTK WQNVLKAHSS121VDWLIVIVEN DAKKKNKTNI LPRTSIVDKI RNDFCNKQSD RCVVLSDPLK DSSRTQESWN181AFLTKLRTLL LMSFTKNLGK FEDDMRTLRE KRTEPGWSFC EYFMVQEELA FVFEMLQQFE241DALVQYDELD ALFSQYVVNF GAGDGANWLT FFCQPVKSWN GLILRKPIDM EKRESIQRRE301ATLLDLRSYL FSRQCTLLLF LQRPWEVAQR ALELLHNCVQ ELKLLEVSVP PGALDCWVFL361SCLEVLQRIE GCCDRAQIDS NIAHTVGLWS YATEKLKSLG YLCGLVSEKG PNSEDLNRTV421DLLAGLGAER PETANTAQSP YKKLKEALSS VEAFEKHYLD LSHATIEMYT SIGRIRSAKF481VGKDLAEFYM RKKAPQKAEI YLQGALKNYL AEGWALPITH TRKQLAECQK HLGQIENYLQ541TSSLLASDHH LTEEERKHFC QEILDFASQP SDSPGHKIVL PMHSFAQLRD LHFDPSNAVV601HVGGVLCVEI TMYSQMPVPV HVEQIVVNVH FSIEKNSYRK TAEWLTKHKT SNGIINFPPE661TAPFPVSQNS LPALELYEMF ERSPSDNSLN TTGIICRNVH MLLRRQESSS SLEMPSGVAL721EEGAHVLRCS HVTLEPGANQ ITFRTQAKEP GTYTLRQLCA SVGSVWFVLP HIYPIVQYDV781YSQEPQLHVE PLADSLLAGI PQRVKFTVTT GHYTIKNGDS LQLSNAEAML ILCQAESRAV841VYSNTREQSS EAALRIQSSD KVTSISLPVA PAYHVIEFEL EVLSLPSAPA LGGESDMLGM901AEPHRKHKDK QRTGRCMVTT DHKVSIDCPW SIYSTVIALT FSVPFRTTHS LLSSGTRKYV961QVCVQNLSEL DFQLSDSYLV DTGDSTDLQL VPLNTQSQQP IYSKQSVFFV WELKWTEEPP1021PSLHCRFSVG FSPASEEQLS ISLKPYTYEF KVENFFTLYN VKAEIFPPSG MEYCRTGSLC1081SLEVLITRLS DLLEVDKDEA LTESDEHFST KLMYEVVDNS SNWAVCGKSC GVISMPVAAR1141ATHRVHMEVM PLFAGYLPLP DVRLFKYLPH HSAHSSQLDA DSWIENDSLS VDKHGDDQPD1201SSSLKSRGSV HSACSSEHKG LPMPRLQALP AGQVFNSSSG TQVLVIPSQD DHVLEVSVTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1259 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.