Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMEFF1 / Tomoregulin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100115-100
Catalog Number: LS-G100115
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEFF1 (NM_003692), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CT120.1 | H7365 | TR-1 | TMEFF1 | C9orf2 | Tomoregulin-1 | Transmembrane protein
- Origin Species
- Hu
- NCBI GenBank
- NM_003692
- SwissProt
- Q8IYR6
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 40.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAAAAEAPL RLPAAPPLAF CCYTSVLLLF AFSLPGSRAS NQPPGGGGGS GGDCPGGKGK61SINCSELNVR ESDVRVCDES SCKYGGVCKE DGDGLKCACQ FQCHTNYIPV CGSNGDTYQN121ECFLRRAACK HQKEITVIAR GPCYSDNGSG SGEGEEEGSG AEVHRKHSKC GPCKYKAECD181EDAENVGCVC NIDCSGYSFN PVCASDGSSY NNPCFVREAS CIKQEQIDIR HLGHCTDTDD241TSLLGKKDDG LQYRPDVKDA SDQREDVYIG NHMPCPENLN GYCIHGKCEF IYSTQKASCR301CESGYTGQHC EKTDFSILYV VPSRQKLTHV LIAAIIGAVQ IAIIVAIVMC ITRKCPKNNR361GRRQKQNLGH FTSDTSSRMVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
380 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.