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TMCO6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMCO6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMCO6 antibodies →
$725.00each
Catalog Number: LS-G111689-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMCO6 (NM_018502), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMCO6 | PRO1580
Origin Species
Human
NCBI GenBank
NM_018502
SwissProt
Q96DC7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWSRRQGRLR PTVCGVEELR RRRREREAAL RKARREQQLV SKRLLRNDAP EEAGEGCVAA
61ILGETEVQQF LRQAQRGTEE KEREGALVSL RRGLQHPETQ QTFIRLEGSM RTLVGLLTSN
121QALLQLEAAR CLHELSHSEQ STVAEACLPA TSYLLTYLSS HSSDFIELCL YTLGNLIVES
181EAVRRQLLPQ GIVPALAACI QSPHVAVLEA LGYALSQLLQ AEEAPEKIIP SILASTLPQH
241MLQMLQPGPK LNPGVAVEFA WCLHYIICSQ VSNPLLIGHG ALSTLGLLLL DLAGAVQKTE
301DAGLELLACP VLRCLSNLLT EAAVETVGGQ MQLRDERVVA ALFILLQFFF QKQPSLLPEG
361LWLLNNLTAN SPSFCTSLLS LDLIEPLLQL LPVSNVVSVM VLTVLCNVAE KGPAYCQRLW
421PGPLLPALLH TLAFSDTEVV GQSLELLHLL FLYQPEAVQV FLQQSGLQAL ERHQEEAQLQ
481DRVYALQQTA LQG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.