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TMCO4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMCO4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMCO4 antibodies →
$504.00each
Catalog Number: LS-G104393-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMCO4 (NM_181719), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RP5-1056L3.6 | TMCO4
Origin Species
Human
SwissProt
Q5TGY1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMWNRPCQR LPQQPLVAEP TAEGEPHLPT GRELTEANRF AYAALCGISL SQLFPEPEHS
61SFCTEFMAGL VQWLELSEAV LPTMTAFASG LGGEGADVFV QILLKDPILK DDPTVITQDL
121LSFSLKDGHY DARARVLVCH MTSLLQVPLE ELDVLEEMFL ESLKEIKEEE SEMAEASRKK
181KENRRKWKRY LLIGLATVGG GTVIGVTGGL AAPLVAAGAA TIIGSAGAAA LGSAAGIAIM
241TSLFGAAGAG LTGYKMKKRV GAIEEFTFLP LTEGRQLHIT IAVTGWLASG KYRTFSAPWA
301ALAHSREQYC LAWEAKYLME LGNALETILS GLANMVAQEA LKYTVLSGIV AALTWPASLL
361SVANVIDNPW GVCLHRSAEV GKHLAHILLS RQQGRRPVTL IGFSLGARVI YFCLQEMAQE
421KDCQGIIEDV ILLGAPVEGE AKHWEPFRKV VSGRIINGYC RGDWLLSFVY RTSSVQLRVA
481GLQPVLLQDR RVENVDLTSV VSGHLDYAKQ MDAILKAVGI RTKPGWDEKG LLLAPGCLPS
541EEPRQAAAAA SSGETPHQVG QTQGPISGDT SKLAMSTDPS QAQVPVGLDQ SEGASLPAAA
601SPERPPICSH GMDPNPLGCP DCACKTQGPS TGLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

634 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.