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TMCC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMCC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TMCC2 antibodies →
$725.00each
Catalog Number: LS-G113652-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMCC2 (NM_014858), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HUCEP11 | TMCC2 | Cerebral protein 11 | KIAA0481
Origin Species
Human
SwissProt
O75069

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MKRCRSDELQ QQQGEEDGAG LEDAASHLPG ADLRPGETTG ANSAGGPTSD AGAAAAPNPG
61PRSKPPDLKK IQQLSEGSMF GHGLKHLFHS RRRSREREHQ TSQDSQQHQQ QQGMSDHDSP
121DEKERSPEMH RVSYAMSLHD LPARPTAFNR VLQQIRSRPS IKRGASLHSS SGGGSSGSSS
181RRTKSSSLEP QRGSPHLLRK APQDSSLAAI LHQHQCRPRS SSTTDTALLL ADGSNVYLLA
241EEAEGIGDKV DKGDLVALSL PAGHGDTDGP ISLDVPDGAP DPQRTKAAID HLHQKILKIT
301EQIKIEQEAR DDNVAEYLKL ANNADKQQVS RIKQVFEKKN QKSAQTIAQL HKKLEHYRRR
361LKEIEQNGPS RQPKDVLRDM QQGLKDVGAN VRAGISGFGG GVVEGVKGSL SGLSQATHTA
421VVSKPREFAS LIRNKFGSAD NIAHLKDPLE DGPPEEAARA LSGSATLVSS PKYGSDDECS
481SASASSAGAG SNSGAGPGGA LGSPKSNALY GAPGNLDALL EELREIKEGQ SHLEDSMEDL
541KTQLQRDYTY MTQCLQEERY RYERLEEQLN DLTELHQNEM TNLKQELASM EEKVAYQSYE
601RARDIQEAVE SCLTRVTKLE LQQQQQQVVQ LEGVENANAR ALLGKFINVI LALMAVLLVF
661VSTIANFITP LMKTRLRITS TTLLVLVLFL LWKHWDSLTY LLEHVLLPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

709 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.