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TMC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106936-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMC4 (NM_144686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TMC4 | Transmembrane channel-like 4
Origin Species
Human
SwissProt
Q7Z404

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEENPTLESE AWGSSRGWLA PREARGGPSL SSVLNELPSA ATLRYRDPGV LPWGALEEEE
61EDGGRSRKAF TEVTQTELQD PHPSRELPWP MQARRAHRQR NASRDQVVYG SGTKTDRWAR
121LLRRSKEKTK EGLRSLQPWA WTLKRIGGQF GAGTESYFSL LRFLLLLNVL ASVLMACMTL
181LPTWLGGAPP GPPGPDISSP CGSYNPHSQG LVTFATQLFN LLSGEGYLEW SPLFYGFYPP
241RPRLAVTYLC WAFAVGLICL LLILHRSVSG LKQTLLAESE ALTSYSHRVF SAWDFGLCGD
301VHVRLRQRII LYELKVELEE TVVRRQAAVR TLGQQARVWL VRVLLNLLVV ALLGAAFYGV
361YWATGCTVEL QEMPLVQELP LLKLGVNYLP SIFIAGVNFV LPPVFKLIAP LEGYTRSRQI
421VFILLRTVFL RLASLVVLLF SLWNQITCGG DSEAEDCKTC GYNYKQLPCW ETVLGQEMYK
481LLLFDLLTVL AVALLIQFPR KLLCGLCPGA LGRLAGTQEF QVPDEVLGLI YAQTVVWVGS
541FFCPLLPLLN TVKFLLLFYL KKLTLFSTCS PAARTFRASA ANFFFPLVLL LGLAISSVPL
601LYSIFLIPPS KLCGPFRGQS SIWAQIPESI SSLPETTQNF LFFLGTQAFA VPLLLISSIL
661MAYTVALANS YGRLISELKR QRQTEAQNKV FLARRAVALT STKPAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

706 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.