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TMC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TMC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107773-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMC2 (NM_080751), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf145 | DJ686C3.3 | TMC2 | Transmembrane channel-like 2
Origin Species
Human
SwissProt
Q8TDI7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSHQVKGLKE EARGGVKGRV KSGSPHTGDR LGRRSSSKRA LKAEGTPGRR GAQRSQKERA
61GGSPSPGSPR RKQTGRRRHR EELGEQERGE AERTCEGRRK RDERASFQER TAAPKREKEI
121PRREEKSKRQ KKPRSSSLAS SASGGESLSE EELAQILEQV EEKKKLIATM RSKPWPMAKK
181LTELREAQEF VEKYEGALGK GKGKQLYAYK MLMAKKWVKF KRDFDNFKTQ CIPWEMKIKD
241IESHFGSSVA SYFIFLRWMY GVNLVLFGLI FGLVIIPEVL MGMPYGSIPR KTVPRAEEEK
301AMDFSVLWDF EGYIKYSALF YGYYNNQRTI GWLRYRLPMA YFMVGVSVFG YSLIIVIRSM
361ASNTQGSTGE GESDNFTFSF KMFTSWDYLI GNSETADNKY ASITTSFKES IVDEQESNKE
421ENIHLTRFLR VLANFLIICC LCGSGYLIYF VVKRSQQFSK MQNVSWYERN EVEIVMSLLG
481MFCPPLFETI AALENYHPRT GLKWQLGRIF ALFLGNLYTF LLALMDDVHL KLANEETIKN
541ITHWTLFNYY NSSGWNESVP RPPLHPADVP RGSCWETAVG IEFMRLTVSD MLVTYITILL
601GDFLRACFVR FMNYCWCWDL EAGFPSYAEF DISGNVLGLI FNQGMIWMGS FYAPGLVGIN
661VLRLLTSMYF QCWAVMSSNV PHERVFKASR SNNFYMGLLL LVLFLSLLPV AYTIMSLPPS
721FDCGPFSGKN RMYDVLQETI ENDFPTFLGK IFAFLANPGL IIPAILLMFL AIYYLNSVSK
781SLSRANAQLR KKIQVLREVE KSHKSVKGKA TARDSEDTPK SSSKNATQLQ LTKEETTPPS
841ASQSQAMDKK AQGPGTSNSA SRTTLPASGH LPISRPPGIG PDSGHAPSQT HPWRSASGKS
901AQRPPH
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

906 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.