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TM9SF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TM9SF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TM9SF4 antibodies →
$725.00each
Catalog Number: LS-G113727-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TM9SF4 (NM_014742), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ836N17.2 | TM9SF4 | KIAA0255
Origin Species
Human
NCBI GenBank
NM_014742
SwissProt
Q92544

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.54 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCETSAFYVP GVAPINFHQN DPVEIKAVKL TSSRTQLPYE YYSLPFCQPS KITYKAENLG
61EVLRGDRIVN TPFQVLMNSE KKCEVLCSQS NKPVTLTVEQ SRLVAERITE DYYVHLIADN
121LPVATRLELY SNRDSDDKKK EKDVQFEHGY RLGFTDVNKI YLHNHLSFIL YYHREDMEED
181QEHTYRVVRF EVIPQSIRLE DLKADEKSSC TLPEGTNSSP QEIDPTKENQ LYFTYSVHWE
241ESDIKWASRW DTYLTMSDVQ IHWFSIINSV VVVFFLSGIL SMIIIRTLRK DIANYNKEDD
301IEDTMEESGW KLVHGDVFRP PQYPMILSSL LGSGIQLFCM ILIVIFVAML GMLSPSSRGA
361LMTTACFLFM FMGVFGGFSA GRLYRTLKGH RWKKGAFCTA TLYPGVVFGI CFVLNCFIWG
421KHSSGAVPFP TMVALLCMWF GISLPLVYLG YYFGFRKQPY DNPVRTNQIP RQIPEQRWYM
481NRFVGILMAG ILPFGAMFIE LFFIFSAIWE NQFYYLFGFL FLVFIILVVS CSQISIVMVY
541FQLCAEDYRW WWRNFLVSGG SAFYVLVYAI FYFVNKLDIV EFIPSLLYFG YTALMVLSFW
601LLTGTIGFYA AYMFVRKIYA AVKID
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

625 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.