Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TLX1 / HOX11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115911-100
Catalog Number: LS-G115911
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HOX11(TLX1) (NM_005521), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Homeobox protein Hox-11 | HOX11 | T-cell leukemia homeobox 1 | TLX1 | Proto-oncogene TCL-3 | TCL3 | T-cell leukemia | homeobox 1
- Origin Species
- Human
- NCBI GenBank
- NM_005521
- SwissProt
- P31314
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEHLGPHHLH PGHAEPISFG IDQILNSPDQ GGCMGPASRL QDGEYGLGCL VGGAYTYGGG61GSAAATGAGG AGAYGTGGPG GPGGPAGGGG ACSMGPLTGS YNVNMALAGG PGPGGGGGSS121GGAGALSAAG VIRVPAHRPL AGAVAHPQPL ATGLPTVPSV PAMPGVNNLT GLTFPWMESN181RRYTKDRFTG HPYQNRTPPK KKKPRTSFTR LQICELEKRF HRQKYLASAE RAALAKALKM241TDAQVKTWFQ NRRTKWRRQT AEEREAERQQ ANRILLQLQQ EAFQKSLAQP LPADPLCVHN301SSLFALQNLQ PWSDDSTKIT SVTSVASACETRTRPLEQKLISEEDLAANDILDYKDDDDKV
330 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.