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TLK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TLK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115039-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TLK2 (NM_006852), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HsHPK | PKU-ALPHA | Tousled-like kinase 2 | TLK2 | Serine/threonine kinase
Origin Species
Human
NCBI GenBank
NM_006852
SwissProt
Q86UE8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMEELHSLDP RRQELLEARF TGVGVSKGPL NSESSNQSLC SVGSLSDKEV ETPEKKQNDQ
61RNRKRKAEPY ETSQGKGTPR GHKISDYFEF AGGSAPGTSP GRSVPPVARS SPQHSLSNPL
121PRRVEQPLYG LDGSAAKEAT EEQSALPTLM SVMLAKPRLD TEQLAQRGAG LCFTFVSAQQ
181NSPSSTGSGN TEHSCSSQKQ ISIQHRQTQS DLTIEKISAL ENSKNSDLEK KEGRIDDLLR
241ANCDLRRQID EQQKMLEKYK ERLNRCVTMS KKLLIEKSKQ EKMACRDKSM QDRLRLGHFT
301TVRHGASFTE QWTDGYAFQN LIKQQERINS QREEIERQRK MLAKRKPPAM GQAPPATNEQ
361KQRKSKTNGA ENETLTLAEY HEQEEIFKLR LGHLKKEEAE IQAELERLER VRNLHIRELK
421RIHNEDNSQF KDHPTLNDRY LLLHLLGRGG FSEVYKAFDL TEQRYVAVKI HQLNKNWRDE
481KKENYHKHAC REYRIHKELD HPRIVKLYDY FSLDTDSFCT VLEYCEGNDL DFYLKQHKLM
541SEKEARSIIM QIVNALKYLN EIKPPIIHYD LKPGNILLVN GTACGEIKIT DFGLSKIMDD
601DSYNSVDGME LTSQGAGTYW YLPPECFVVG KEPPKISNKV DVWSVGVIFY QCLYGRKPFG
661HNQSQQDILQ ENTILKATEV QFPPKPVVTP EAKAFIRRCL AYRKEDRIDV QQLACDPYLL
721PHIRKSVSTS SPAGAAIAST SGASNNSSSN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

750 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.