LSBio
TLE3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TLE3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G124100-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TLE3 (NM_001105192), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ESG | ESG3 | Enhancer of split groucho 3 | HsT18976 | KIAA1547 | GRG3 | TLE3
Origin Species
Human
NCBI GenBank
NM_005078
SwissProt
Q04726

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYPQGRHPAP HQPGQPGFKF TVAESCDRIK DEFQFLQAQY HSLKVEYDKL ANEKTEMQRH
61YVMYYEMSYG LNIEMHKQTE IAKRLNTILA QIMPFLSQEH QQQVAQAVER AKQVTMTELN
121AIIGQQQLQA QHLSHATHGP PVQLPPHPSG LQPPGIPPVT GSSSGLLALG ALGSQAHLTV
181KDEKNHHELD HRERESSANN SVSPSESLRA SEKHRGSADY SMEAKKRKAE EKDSLSRYDS
241DGDKSDDLVV DVSNEDPATP RVSPAHSPPE NGLDKARSLK KDAPTSPASV ASSSSTPSSK
301TKDLGHNDKS STPGLKSNTP TPRNDAPTPG TSTTPGLRSM PGKPPGMDPI ASALRTPISI
361TSSYAAPFAM MSHHEMNGSL TSPGAYAGLH NIPPQMSAAA AAAAAAYGRS PMVSFGAVGF
421DPHPPMRATG LPSSLASIPG GKPAYSFHVS ADGQMQPVPF PHDALAGPGI PRHARQINTL
481SHGEVVCAVT ISNPTRHVYT GGKGCVKIWD ISQPGSKSPI SQLDCLNRDN YIRSCKLLPD
541GRTLIVGGEA STLTIWDLAS PTPRIKAELT SSAPACYALA ISPDAKVCFS CCSDGNIAVW
601DLHNQTLVRQ FQGHTDGASC IDISHDGTKL WTGGLDNTVR SWDLREGRQL QQHDFTSQIF
661SLGYCPTGEW LAVGMESSNV EVLHHTKPDK YQLHLHESCV LSLKFAYCGK WFVSTGKDNL
721LNAWRTPYGA SIFQSKESSS VLSCDISADD KYIVTGSGDK KATVYEVIY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

769 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.