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TKTL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TKTL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109023-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TKTL2 (NM_032136), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Transketolase-like protein 2 | Transketolase-like 2 | TKTL2 | DKFZP434L1717
Origin Species
Human
NCBI GenBank
NM_032136
SwissProt
Q9H0I9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMANDAKPDV KTVQVLRDTA NRLRIHSIRA TCASGSGQLT SCCSAAEVVS VLFFHTMKYK
61QTDPEHPDND RFILSRGHAA PILYAAWVEV GDISESDLLN LRKLHSDLER HPTPRLPFVD
121VATGSLGQGL GTACGMAYTG KYLDKASYRV FCLMGDGESS EGSVWEAFAF ASHYNLDNLV
181AVFDVNRLGQ SGPAPLEHGA DIYQNCCEAF GWNTYLVDGH DVEALCQAFW QASQVKNKPT
241AIVAKTFKGR GIPNIEDAEN WHGKPVPKER ADAIVKLIES QIQTNENLIP KSPVEDSPQI
301SVTDIKMTSP HAYKVGDKIA TQKTYGLALA KLGRANERVI VLSGDTMNST FSEIFRKEHP
361ERFIECIIAE QNMVSVALGC ATRGRTIAFA GAFAAFFTRA FDQLRMGAIS QANINLIGSH
421CGVSTGEDGV SQMALEDLAM FRSIPNCTVF YPSDAISTEH AIYLAANTKG MCFIRTSQPE
481TAVIYTPQEN FEIGQAKVVR HGVNDKVTVI GAGVTLHEAL EAADHLSQQG ISVRVIDPFT
541IKPLDAATII SSAKATGGRV ITVEDHYREG GIGEAVCAAV SREPDILVHQ LAVSGVPQRG
601KTSELLDMFG ISTRHIIAAV TLTLMK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.