Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TIP49 / RUVBL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117144-100
Catalog Number: LS-G117144
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TIP49A(RUVBL1) (NM_003707), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 49 kDa TBP-interacting protein | ECP54 | INO80 complex subunit H | INO80H | Nuclear matrix protein 238 | Pontin52 | RuvB-like 1 | RUVBL1 | TIH1 | TAP54-alpha | TIP49 | NMP 238 | PONTIN | RuvB (E coli homolog)-like 1 | RuvB-like 1 (E. coli) | ECP-54 | NMP238 | Pontin 52 | RVB1 | TIP49A
- Origin Species
- Human
- NCBI GenBank
- NM_003707
- SwissProt
- Q9Y265
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIEEVKSTT KTQRIASHSH VKGLGLDESG LAKQAASGLV GQENAREACG VIVELIKSKK61MAGRAVLLAG PPGTGKTALV LAIAQELGSK VPFCPMVGSE VYSTEIKKTE VLMENFRRAI121GLRIKETKEV YEGEVTELTP CETENPMGGY GKTISHVIIG LKTAKGTKQL KLDPSIFESL181QKERVEAGDV IYIEANSGAV KRQGRCDTYA TEFDLEAEEY VPLPKGDVHK KKEIIQDVTL241HDLDVANARP QGGQDILSMM GQLMKPKKTE ITDKLRGEIN KVVNKYIDQG IAELVPGVLF301VDEVHMLDIE CFTYLHRALE SSIAPIVIFA SNRGNCVIRG TEDITSPHGI PLDLLDRVMI361IRTMLYTPQE MKQIIKIRAQ TEGINISEEA LNHLGEIGTK TTLRYSVQLL TPANLLAKIN421GKDSIEKEHV EEISELFYDA KSSAKILADQ QDKYMKTRTR PLEQKLISEE DLAANDILDY481KDDDDKV
487 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.