Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TINAGL1 / Lipocalin 7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110405-100
Catalog Number: LS-G110405
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TINAGL1 (NM_022164), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LIECG3 | OLRG2 | OLRG-2 | Oxidized-LDL responsive gene 2 | p3ECSL | Androgen-regulated gene 1 | Lipocalin 7 | TIN-Ag-RP | TINAG-like 1 | TINAGL | TINAGL1 | TINAGRP | GIS5 | LCN7 | TIN Ag-related protein
- Origin Species
- Human
- NCBI GenBank
- AF236150
- SwissProt
- Q9GZM7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MWRCPLGLLL LLPLAGHLAL GAQQGRGRRE LAPGLHLRGI RDAGGRYCQE QDLCCRGRAD61DCALPYLGAI CYCDLFCNRT VSDCCPDFWD FCLGVPPPFP PIQGCMHGGR IYPVLGTYWD121NCNRCTCQEN RQWQCDQEPC LVDPDMIKAI NQGNYGWQAG NHSAFWGMTL DEGIRYRLGT181IRPSSSVMNM HEIYTVLNPG EVLPTAFEAS EKWPNLIHEP LDQGNCAGSW AFSTAAVASD241RVSIHSLGHM TPVLSPQNLL SCDTHQQQGC RGGRLDGAWW FLRRRGVVSD HCYPFSGRER301DEAGPAPPCM MHSRAMGRGK RQATAHCPNS YVNNNDIYQV TPVYRLGSND KEIMKELMEN361GPVQALMEVH EDFFLYKGGI YSHTPVSLGR PERYRRHGTH SVKITGWGEE TLPDGRTLKY421WTAANSWGPA WGERGHFRIV RGVNECDIES FVLGVWGRVG MEDMGHHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
467 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.